New research reveals how genes turn on and off

يكشف بحث جديد كيف تعمل الجينات وتتوقف Genes and Development (2022). DOI: 10.1101 / gad.350026.122″ width=”800″ height=”530″/>

Protein architectures of constitutive and inducible (RP, induced, balanced, and condition-specific) promoters. Compound plots of chromatin immunoprecipitation exonuclease (ChIP-exo) tag 5 (exonuclease stop sites as shown) are shown for 70 representative gene-associated proteins (out of 400) distributed around the reference point of the indicated X axis (UAS, Sua7, or +1 nuc) within each promoter class (rows of panels). Each target protein is identified in a single color vertical column with the legend at the bottom. The selected complexes represented by the protein are indicated in the given panels. See Materials and Methods for details. X-axis intervals are oriented so that transcription continues to the right. The UAS corresponds to the ssTF binding site or the equivalent distance upstream (150 bp) to the transcription start site (TSS) in the absence of UAS. Sua7 (TFIIB) corresponds to the Sua7 ChExMix peak closest to the annotated TSS and is essentially the PIC site. +1 nuc is the midpoint of the nucleosome downstream closest to the TSS. Alignment of these reference features provided high position accuracy. The corresponding bar data is reversed. “N” indicates the number of class membership. The measure of Y-axis occupancy is the same and therefore comparable for a given target across the different promoter classes (analysis ID: CM701- CM707). attributed to him: Genes and Development (2022). DOI: 10.1101 / gad.350026.122

Yeast, that simple organism essential to making beer and bread, revealed to Cornell University researchers a key mechanism in how genes are controlled.

Gene transcription – the complex process our cells use to read genetic information Stored in DNA – It has long been thought to be triggered only when certain regulatory factors are transcribed into specific DNA sequences. In new research, a team of Cornell scientists has discovered this for sure genes It has transcription-regulatory factors and cofactors already present, but in a latent state. With appropriate signals, these “stable” genes become highly active.

Using CRISPR techniques, the researchers removed parts of the yeast transcription machinery to systematically examine the role it plays in gene regulation. Yeast and humans often have the same molecular mechanism for regulating their genes, so yeast provides an excellent model for understanding Gene regulation in humans.

Mold. Franklin Pogue, professor of molecular biology and genetics and corresponding author of the study.

“The value of predisposition is that some genes, such as environmental response genes, can respond quickly to a changing environment; for example, when yeast It encounters and metabolizes the bread sugars, which causes the bread dough to rise.”

“Building on years of existing research and combining it with modern and elegant genomics tools has helped us fill in gaps in current knowledge as well as make new discoveries,” said Shitvan Mittal, first author and research associate at the Baker Institute for Animal Health. in the College of Veterinary Medicine.

The search was published in Genes and Development.

Yeast epigenome map reveals details of gene regulation

more information:
Chitvan Mittal et al, Integrated selectively integrated SAGA and TFIID PIC assembly pathway for balanced and induced promoters, Genes and Development (2022). DOI: 10.1101 / gad.350026.122

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